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Wuehlisch, George Von
- RAPD Analysis of Induced Mutants of Jatropha (Jatropha curcas)
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Authors
Affiliations
1 CCS Haryana Agricultural University, Hisar-125004, IN
2 Federal Research Institute for Rural Areas, Forestry and Fisheries, Institute for Forest Genetics, Grosshansdorf, DE
3 Central Soil and Water Conservation Research and Training Institute, Dehradun, IN
1 CCS Haryana Agricultural University, Hisar-125004, IN
2 Federal Research Institute for Rural Areas, Forestry and Fisheries, Institute for Forest Genetics, Grosshansdorf, DE
3 Central Soil and Water Conservation Research and Training Institute, Dehradun, IN
Source
Indian Forester, Vol 139, No 12 (2013), Pagination: 1097-1104Abstract
Genetic variability in Jatropha curcas was induced by different doses (5, 10, 15, 20, 25, 30, 35, 40, 45 and 50 kR) of gamma-rays. Gamma radiation induced earliness in flowering and the plants set flowers earlier than that of normal (control), which took longer duration of 327 days for flowering. The improved reproductive and yield parameters such as days taken to first flowering, flowering population, male to female ratio and seed yield per plant were recorded in 25 kR dose and seed germination in 5 and 10 kR treated seeds. Molecular characterization of induced mutants (M1 generation) with 47 RAPD primers showed 65.27 per cent polymorphism. The variability created by gamma rays ranged from 9 to 28 per cent. The 50 kR mutant was found to be the most diverse from control followed by 25 kR mutant. Thus, this integrated approach can be used for carrying out the mutation-assisted breeding and subsequent selection of desired mutants using molecular markers in J. curcas.Keywords
Jatropha curcas, Gamma-rays, Induced Mutagenesis, RAPD
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